High-performance liquid chromatography (HPLC) is an analytical testing instrument that is used globally to provide specific information about the components of a liquid solution. It is called high performance because it accepts tinier particles as material for packing the column. A related type is ultra-high-performance liquid chromatography (UHPLC).
The Basic Method
The base analysis of a product or related item using this method requires two major components:
1. A liquid sample
2. A solvent
The compound is to be dissolved only into a solvent. Other necessary equipment in high-performance liquid chromatography consists of a solvent delivery pump,a detector and a column. These are valid whether the system represents the earliest form of HPLC – high-pressure liquid chromatography, or the more sophisticated and modernized variation.
The Three Goals
High-performance liquid chromatography is a method employed to accomplish three goals regarding the components of liquid samples. These are:
1. Separation: The term for this is chromatography. In HPLC, the stationary phase has a larger surface area. This produces a higher quality of separation
2. Identification: Chromatography separates the particles in the liquid allowing for identification
3. Quantification: The same method is responsible for any
These three goals are a perfect example of both qualitative and quantitative analysis. They not only identify the components but provide a thorough analysis of eth amount of each component within the liquid sample. When laboratories use High-performance liquid chromatography the results are better than in other methods and the analysis is easier.
High-Performance Liquid Chromatography
HPLC is not the only method available for separating, identifying and quantifying samples. However, because the results are rapid and accurate, it remains a preferred method in many medical and pharmaceutical industries. Many companies can provide industrial and commercial concerns with HPLC equipment. However, if you are concerned with quality and precision, contact professionals at Trinity Biotech. They can help you find the exacting tools your company requires to conduct the necessary tasks swiftly and accurately.
